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ProductsBack/Complement C3 Polyclonal Antibody
Formalin-fixed and paraffin embedded rat liver labeled with Anti-C3 Polyclonal Antibody, Unconjugated (bs-2934R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37°C
Formalin-fixed and paraffin embedded rat liver labeled with Anti-C3 Polyclonal Antibody, Unconjugated (bs-2934R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37°C
Formalin-fixed and paraffin embedded rat liver labeled with Anti-C3 Polyclonal Antibody, Unconjugated (bs-2934R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated(bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37°C

Complement C3 Polyclonal Antibody

BS-2934R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetC3
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Overview

  • Supplier
    Bioss
  • Product Name
    Complement C3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID718
  • Target name
    C3
  • Target description
    complement C3
  • Target synonyms
    AHUS5, ARMD9, ASP, C3a, C3b, CPAMD1, HEL-S-62p, complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, C3a anaphylatoxin, acylation-stimulating protein cleavage product, complement component 3, complement component C3a, complement component C3b, epididymis secretory sperm binding protein Li 62p, prepro-C3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01024
  • Protein Name
    Complement C3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The proteasome activator REGgamma counteracts immunoproteasome expression and autoimmunity. Yao L et al., 2019 Sep, J Autoimmun
    Read more
  • Immunosuppressive effect of artemisinin and hydroxychloroquine combination therapy on IgA nephropathy via regulating the differentiation of CD4+ T cell subsets in rats. Bai L et al., 2019 May, Int Immunopharmacol
    Read more
  • Depletion of the Third Complement Component Ameliorates Age-Dependent Oxidative Stress and Positively Modulates Autophagic Activity in Aged Retinas in a Mouse Model. Roginska D et al., 2017, Oxid Med Cell Longev
    Read more
  • A Novel Mechanism for Generating the Interferon Signature in Lupus: Opsonization of Dead Cells by Complement and IgM. Zhuang H et al., 2016 Dec, Arthritis Rheumatol
    Read more
  • Tacrolimus Protects Podocytes from Injury in Lupus Nephritis Partly by Stabilizing the Cytoskeleton and Inhibiting Podocyte Apoptosis. Liao R et al., 2015, PLoS One
    Read more

Datasheet

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Figure 1. Western blot analysis of C3 using anti-C3 antibody (A00168-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat plasma lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse plasma lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C3 antigen affinity purified polyclonal antibody (Catalog # A00168-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C3 at approximately 128KD/187KD. The expected band size for C3 is at 187KD.
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