Anti-C3b-iC3b [7C12]

Absolute Antibody

Anti-C3b-iC3b [7C12]

7

To prepare and characterize this antibody, an ELISA on iC3 protein was performed using the mouse version of this antibody (Tosic et al, 1989; pmid:2786912). An immunoblot was performed on control, early AD, and AD patient brain tissues using the mouse version of this antibody to determine the link between the complement system and Alzheimers disease (Wu et al, 2019; pmid:31433986). To study the deposition of the complement protein fragment C3b and its breakdown products on CD20-positive cells, flow cytometry was performed on Raji or ARH-77 cells using the mouse version of this antibody. Furthermore, a radioimmunoassay was performed on Raji or ARH-77 cells using this antibody. Finally, Raji or ARH-77 cells were stained for immunofluorescence using this antibody (Kennedy et al, 2003; pmid:12393727). To study how Rituximab activates complement and promotes covalent deposition of C3 fragments (C3b/iC3b) on cells, flow cytometry was performed on raji cells in presence of the mouse version of this antibody. Furthermore, raji cells were stained for immunofluorescence using this antibody (Beum et al, 2006; pmid:17067631). To study the alternative pathway (AP) of the complement system, flow cytometry was performed on AP-mediated C3b-opsonizated zymosan using the mouse version of this antibody. Furthermore, immunofluorescence was performed on AP-mediated C3b-opsonizated zymosan using this antibody (DiLillo et al, 2006; pmid:15961157).

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA

ELISA; WB; IF; FC

Research Use Only

Monoclonal

7C12

C3

complement C3

acylation-stimulating protein cleavage product; AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a; C3a anaphylatoxin; C3b; complement C3; complement component 3; complement component C3a; complement component C3b; CPAMD1; epididymis secretory sperm binding protein Li 62p; HEL-S-62p; prepro-C3

Mouse

IgG1

Complement C3

Human

The mouse version of this antibody was raised by immunizing a mouse with human C3b and iC3b bound to a carbohydrate matrix via the alternative pathway of complement activation.

-20°C,2°C to 8°C

12352203