ChIP analysis of 2 million HeLa cells treated with 50ng/ml of TNF alpha for one hour using GTX10564 CREB (phospho Ser129/Ser133) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of I-kB, YWHAZ gene as positive control target and the inactive GAPDH used as negative control target.
ChIP analysis of 2 million HeLa cells treated with 50ng/ml of TNF alpha for one hour using GTX10564 CREB (phospho Ser129/Ser133) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of I-kB, YWHAZ gene as positive control target and the inactive GAPDH used as negative control target.
CREB (phospho Ser129/Ser133) antibody
GTX10564
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCREB1
Overview
- SupplierGeneTex
- Product NameCREB (phospho Ser129/Ser133) antibody - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:200-1:2000. ICC/IF: 1:100-1:500. ChIP assay: 10microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID1385
- Target nameCREB1
- Target descriptioncAMP responsive element binding protein 1
- Target synonymsactive transcription factor CREB; cAMP-response element-binding protein-1; CREB; CREB-1; cyclic adenosine 3',5'-monophosphate response element binding protein; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; transactivator protein
- HostRabbit
- IsotypeIgG
- Protein IDP16220
- Protein NameCyclic AMP-responsive element-binding protein 1
- Scientific DescriptionThis gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203