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CRX Rabbit pAb

A5719
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCRX
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Overview

  • Supplier
    ABclonal
  • Product Name
    CRX Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    WB,IF/ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1406
  • Target name
    CRX
  • Target description
    cone-rod homeobox
  • Target synonyms
    CORD2, CRD, LCA7, OTX3, cone-rod homeobox protein, orthodenticle homeobox 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43186
  • Protein Name
    Cone-rod homeobox protein
  • Reactivity
    Human, Mouse
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CORD2 using anti-CORD2 antibody (A02202-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CORD2 antigen affinity purified polyclonal antibody (Catalog # A02202-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CORD2 at approximately 37KD. The expected band size for CORD2 is at 32KD.
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