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Western Blot Positive WB detected in: Rat heart tissue, Mouse brain tissue All lanes: CTH antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 40, 42 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: Rat heart tissue, Mouse brain tissue All lanes: CTH antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 40, 42 kDa Observed band size: 45 kDa
Western Blot Positive WB detected in: Rat heart tissue, Mouse brain tissue All lanes: CTH antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 40, 42 kDa Observed band size: 45 kDa

CTH Antibody

CSB-PA006160LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCTH
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Overview

  • Supplier
    Cusabio
  • Product Name
    CTH Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1491
  • Target name
    CTH
  • Target description
    cystathionine gamma-lyase
  • Target synonyms
    CSE; cystathionase (cystathionine gamma-lyase); cystathionine gamma-lyase; cysteine desulfhydrase; cysteine-protein sulfhydrase; gamma-cystathionase; homoserine deaminase; homoserine dehydratase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32929
  • Protein Name
    Cystathionine gamma-lyase
  • Scientific Description
    Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cystathionase using anti-Cystathionase antibody (PB9494). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: K562 whole cell lysates, lane 2: HepG2 whole cell lysates, lane 3: RH35 whole cell lysates, lane 4: rat liver tissue lysates, lane 5: rat kidney tissue lysates, lane 6: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystathionase antigen affinity purified polyclonal antibody (Catalog # PB9494) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystathionase at approximately 45KD. The expected band size for Cystathionase is at 45KD.
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