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Cystathionase Antibody (YA2452, Rabbit)

HY-P82707
MedChem Express
TargetCTH
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Cystathionase Antibody (YA2452, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1491
  • Target name
    CTH
  • Target description
    cystathionine gamma-lyase
  • Target synonyms
    CGL, CSE, cystathionine gamma-lyase, cystathionase (cystathionine gamma-lyase), cysteine desulfhydrase, cysteine-protein sulfhydrase, gamma-cystathionase, homocysteine desulfhydrase, homoserine deaminase, homoserine dehydratase
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Cystathionase Antibody (YA2452) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2452), targeting Cystathionase, with a predicted molecular weight of 45 kDa (observed band size: 42 kDa). Cystathionase Antibody (YA2452) can be used for WB, IP experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cystathionase using anti-Cystathionase antibody (PB9494). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: K562 whole cell lysates, lane 2: HepG2 whole cell lysates, lane 3: RH35 whole cell lysates, lane 4: rat liver tissue lysates, lane 5: rat kidney tissue lysates, lane 6: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystathionase antigen affinity purified polyclonal antibody (Catalog # PB9494) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystathionase at approximately 45KD. The expected band size for Cystathionase is at 45KD.
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