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CTH antibody

GTX113409
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCTH
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Overview

  • Supplier
    GeneTex
  • Product Name
    CTH antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.02 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1491
  • Target name
    CTH
  • Target description
    cystathionine gamma-lyase
  • Target synonyms
    CSE; cystathionase (cystathionine gamma-lyase); cystathionine gamma-lyase; cysteine desulfhydrase; cysteine-protein sulfhydrase; gamma-cystathionase; homoserine deaminase; homoserine dehydratase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32929
  • Protein Name
    Cystathionine gamma-lyase
  • Scientific Description
    This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sorafenib fails to trigger ferroptosis across a wide range of cancer cell lines. Zheng J et al., 2021 Jul 13, Cell Death Dis
    Read more
  • High-throughput analyses of hnRNP H1 dissects its multi-functional aspect. Uren PJ et al., 2016, RNA Biol
    Read more

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Figure 1. Western blot analysis of Cystathionase using anti-Cystathionase antibody (PB9494). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: K562 whole cell lysates, lane 2: HepG2 whole cell lysates, lane 3: RH35 whole cell lysates, lane 4: rat liver tissue lysates, lane 5: rat kidney tissue lysates, lane 6: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cystathionase antigen affinity purified polyclonal antibody (Catalog # PB9494) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cystathionase at approximately 45KD. The expected band size for Cystathionase is at 45KD.
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