![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody [HL2732] (GTX639560) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody [HL2732] (GTX639560) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX639560/GTX639560_45362_20240329_WB_B_24041019_554.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody [HL2732] (GTX639560) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CTLA4 antibody [HL2732]
GTX639560
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCtla4
Overview
- SupplierGeneTex
- Product NameCTLA4 antibody [HL2732]
- Delivery Days Customer9
- Application Supplier NoteIHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2732
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID12477
- Target nameCtla4
- Target descriptioncytotoxic T-lymphocyte-associated protein 4
- Target synonymsCd152, Ctla-4, Ly-56, cytotoxic T-lymphocyte protein 4, CD152 antigen, cytotoxic T-lymphocyte-associated antigen 4
- HostRabbit
- IsotypeIgG
- Protein IDP09793
- Protein NameCytotoxic T-lymphocyte protein 4
- Scientific DescriptionThis gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![CTLA4 antibody [HL2732] detects CTLA4 protein by immunohistochemical analysis. Sample: Paraffin-embedded human spleen. CTLA4 stained by CTLA4 antibody [HL2732] (GTX639560) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min CTLA4 antibody [HL2732] detects CTLA4 protein by immunohistochemical analysis. Sample: Paraffin-embedded human spleen. CTLA4 stained by CTLA4 antibody [HL2732] (GTX639560) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX639560/GTX639560_T-45264_20240531_IHC-P_1_24060619_820.webp)
![NCI-H520 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody [HL2732] (GTX639560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract) NCI-H520 whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody [HL2732] (GTX639560) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)](https://www.genetex.com/upload/website/prouct_img/normal/GTX639560/GTX639560_45362_20240719_WB_Fraction_24072519_124.webp)



![WB analysis of purified CTLA4 recombinant protein using GTX13996 CTLA4 antibody [UC10-4F10-11]. Lane 1 : 1 ug reduced purified mouse CTLA-4 with histidine tag 0.5 ug reduced purified mouse Dilution : 15 ug/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX13996/GTX13996_20200917_WB_w_23060620_532.webp)