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CTLA4 antibody

GTX13486
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
TargetCtla4
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Overview

  • Supplier
    GeneTex
  • Product Name
    CTLA4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.2 microg/ml. ELISA: 0.1-0.4 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID12477
  • Target name
    Ctla4
  • Target description
    cytotoxic T-lymphocyte-associated protein 4
  • Target synonyms
    Cd152, Ctla-4, Ly-56, cytotoxic T-lymphocyte protein 4, CD152 antigen, cytotoxic T-lymphocyte-associated antigen 4
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP09793
  • Protein Name
    Cytotoxic T-lymphocyte protein 4
  • Scientific Description
    CTLA4 has a critical role in T cell differentiation and contributes to the maintenance of peripheral T cell tolerance to self antigens. It exerts its major effects on T cell immune responses via regulation of the cell cycle and may be involved in T-cell activation. It binds to B7-1 (CD80) and B7-2 (CD86), and it contains 1 immunoglobulin-like V-type domain. Widely expressed with highest levels in lymphoid tissues.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CTLA4 using anti-CTLA4 antibody (A00020-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse NIH3T3 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTLA4 antigen affinity purified polyclonal antibody (Catalog # A00020-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTLA4 at approximately 25KD. The expected band size for CTLA4 is at 25KD.
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