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CUL1 / Cullin 1 Antibody

LS-C331929
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCUL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CUL1 / Cullin 1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 89kDa, while the observed MW by Western blot was 100kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:10 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 89kDa, while the observed MW by Western blot was 100kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8454
  • Target name
    CUL1
  • Target description
    cullin 1
  • Target synonyms
    CUL-1; cullin-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cullin 1 using anti-Cullin 1 antibody (PB9542). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Spleen Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: Rat Thymus Tissue Lysate at 50ug, Lane 4: Mouse Lung Tissue Lysate at 50ug, Lane 5: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 1 antigen affinity purified polyclonal antibody (Catalog # PB9542) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 1 at approximately 90 kDa. The expected band size for Cullin 1 is at 90 kDa.
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