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Cullin 1 Antibody (YA2378, Rabbit)

HY-P82633
MedChem Express
TargetCUL1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Cullin 1 Antibody (YA2378, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8454
  • Target name
    CUL1
  • Target description
    cullin 1
  • Target synonyms
    cullin-1, CUL-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Cullin 1 Antibody (YA2378) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cullin 1.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cullin 1 antibody [JM72-30] (GTX00988) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of Cullin 1 using anti-Cullin 1 antibody (PB9542). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Spleen Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: Rat Thymus Tissue Lysate at 50ug, Lane 4: Mouse Lung Tissue Lysate at 50ug, Lane 5: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 1 antigen affinity purified polyclonal antibody (Catalog # PB9542) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 1 at approximately 90 kDa. The expected band size for Cullin 1 is at 90 kDa.
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