ICC/IF analysis of U-87 MG cells treated 1 uM thapsigargin for 24 hours using GTX15763 CXCL8 / IL8 antibody [3IL8-H10]. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute
![Sandwich ELISA analysis of serial diluted human IL-8 recombinant protein(50ul per well) using GTX15763 CXCL8 / IL8 antibody [3IL8-H10] (capture antibody) at 3 microg/mL and biotinylated mouse anti-human IL-8 monoclonal antibody (detection antibody) at 0.25 microg/mL.](https://www.genetex.com/upload/website/prouct_img/normal/GTX15763/GTX15763_65_ELISA_w_23060620_916.webp "Sandwich ELISA analysis of serial diluted human IL-8 recombinant protein(50ul per well) using GTX15763 CXCL8 / IL8 antibody [3IL8-H10] (capture antibody) at 3 microg/mL and biotinylated mouse anti-human IL-8 monoclonal antibody (detection antibody) at 0.25 microg/mL.")
ICC/IF analysis of U-87 MG cells treated 1 uM thapsigargin for 24 hours using GTX15763 CXCL8 / IL8 antibody [3IL8-H10]. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute
CXCL8 / IL8 antibody [3IL8-H10]
GTX15763
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCXCL8
Overview
- SupplierGeneTex
- Product NameCXCL8 / IL8 antibody [3IL8-H10] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 2 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3IL8-H10
- Concentration1.08 mg/ml
- ConjugateUnconjugated
- Gene ID3576
- Target nameCXCL8
- Target descriptionC-X-C motif chemokine ligand 8
- Target synonymsalveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; chemokine (C-X-C motif) ligand 8; emoctakin; GCP1; GCP-1; granulocyte chemotactic protein 1; IL8; interleukin 8; interleukin-8; LECT; LUCT; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; LYNAP; MDNCF; MONAP; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; NAF; NAP1; NAP-1; neutrophil-activating peptide 1; SCYB8; small inducible cytokine subfamily B, member 8; T-cell chemotactic factor; tumor necrosis factor-induced gene 1
- HostMouse
- IsotypeIgG
- Protein IDP10145
- Protein NameInterleukin-8
- Scientific DescriptionThe protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is secreted primarily by neutrophils, where it serves as a chemotactic factor by guiding the neutrophils to the site of infection. This chemokine is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, Aug 2017]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203