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WB analysis of HeLa whole cell lysates using GTX27957 Cyclin B1 antibody.
WB analysis of HeLa whole cell lysates using GTX27957 Cyclin B1 antibody.
WB analysis of HeLa whole cell lysates using GTX27957 Cyclin B1 antibody.

Cyclin B1 antibody

GTX27957
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetCCNB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Cyclin B1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID891
  • Target name
    CCNB1
  • Target description
    cyclin B1
  • Target synonyms
    CCNB; G2/mitotic-specific cyclin-B1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14635
  • Protein Name
    G2/mitotic-specific cyclin-B1
  • Scientific Description
    The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • The BRG1- and hBRM-associated factor BAF57 induces apoptosis by stimulating expression of the cylindromatosis tumor suppressor gene. Wang L et al., 2005 Sep, Mol Cell Biol
    Read more

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Figure 1. Western blot analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # A00745-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.
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