More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Cyclin B1 antibody

Cyclin B1 antibody

GTX73034
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCCNB1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Cyclin B1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    For IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID891
  • Target name
    CCNB1
  • Target description
    cyclin B1
  • Target synonyms
    CCNB; G2/mitotic-specific cyclin-B1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14635
  • Protein Name
    G2/mitotic-specific cyclin-B1
  • Scientific Description
    The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
Anti-CCNB1 Antibody144-02056
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetCCNB1
  • SizePrice
WB analysis of HeLa whole cell lysates using GTX27957 Cyclin B1 antibody.
Product group Antibodies
References
GeneTex
Cyclin B1 antibodyGTX27957
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetCCNB1
  • SizePrice
Western blot analysis of Cyclin B1 in Cos17 cell extract using GTX30914.
Product group Antibodies
GeneTex
Cyclin B1 antibody [GNS1]GTX30914
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
TargetCCNB1
  • SizePrice
Figure 1. Western blot analysis of CCNB1 using anti-CCNB1 antibody (A00745-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNB1 antigen affinity purified polyclonal antibody (Catalog # A00745-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNB1 at approximately 55KD. The expected band size for CCNB1 is at 55KD.
Product group Antibodies
Boster Bio
Anti-Cyclin B1/CCNB1 Antibody Picoband(r)A00745-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCCNB1
  • SizePrice
Product group Antibodies
Biomatik
Cyclin B (CCNB) Polyclonal AntibodyCAU23450
ApplicationsWestern Blot
TargetCCNB1
  • SizePrice
Product group Antibodies
Assay Genie
Cyclin B1 AntibodyCAB16038
TargetCCNB1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded Human lung Tissue using Cyclin B1 Mouse mAb diluted at 1:200.
Product group Antibodies
Cusabio
CCNB1 Monoclonal AntibodyCSB-MA124445
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCCNB1
  • SizePrice
HeLa lysates probed with Anti –(Name) Polyclonal Antibody, Unconjugated (Catalog #) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (Secondary Catalog #) at 1:5000 for 90 min at 37˚C.
Product group Antibodies
References
Bioss Antibodies
Cyclin B1 Polyclonal AntibodyBS-0572R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCCNB1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-Cyclin B1/CCNB1 Antibody, Affinity PurifiedA305-000A
ApplicationsImmunoPrecipitation, Western Blot
TargetCCNB1
  • SizePrice
Product group Antibodies
MedChem Express
Cyclin B1 Antibody (YA486, Rabbit)HY-P80097
TargetCCNB1
  • SizePrice