Western blot analysis is shown using GeneTex Anti-Cyclin D1 antibody (GTX21224) to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG GTX27090 and chemiluminescence reagent with a 1min exposure time. Other detection systems will yield similar results.
Western blot analysis is shown using GeneTex Anti-Cyclin D1 antibody (GTX21224) to detect Human Cyclin D1 present in asynchronous HN30 cell lysates. HN30 cells, are from head and neck cancer cells that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~34 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG GTX27090 and chemiluminescence reagent with a 1min exposure time. Other detection systems will yield similar results.
Cyclin D1 antibody
GTX21224
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCCND1
Overview
- SupplierGeneTex
- Product NameCyclin D1 antibody
- Delivery Days Customer9
- Antibody SpecificityNo reaction was observed against other related cyclins.
- Application Supplier NoteWB: 1:500-1:1000. IP: 1:100. ELISA: 1:2000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoPrecipitation, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID595
- Target nameCCND1
- Target descriptioncyclin D1
- Target synonymsB-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL1; BCL-1 oncogene; D11S287E; G1/S-specific cyclin-D1; PRAD1; PRAD1 oncogene; U21B31
- HostRabbit
- IsotypeIgG
- Protein IDP24385
- Protein NameG1/S-specific cyclin-D1
- Scientific DescriptionCyclin D1 (also known as G1/S-specific cyclin D1, PRAD1 oncogene, BCL-1 oncogene, and PRAD1: parathyroid adenomatosis 1) is encoded by a gene that belongs to the highly conserved cyclin family. Cyclins are characterized by a dramatic periodicity in protein abundance throughout the cell cycle and function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203