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WB analysis of rat testis tissue lysate using GTX33134 CYP17A1 antibody. Dilution : 1:500 Loading : 25microg per lane
WB analysis of rat testis tissue lysate using GTX33134 CYP17A1 antibody. Dilution : 1:500 Loading : 25microg per lane
WB analysis of rat testis tissue lysate using GTX33134 CYP17A1 antibody. Dilution : 1:500 Loading : 25microg per lane

CYP17A1 antibody

GTX33134
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetCYP17A1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CYP17A1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1586
  • Target name
    CYP17A1
  • Target description
    cytochrome P450 family 17 subfamily A member 1
  • Target synonyms
    17-alpha-hydroxyprogesterone aldolase; CPT7; CYP17; CYPXVII; cytochrome P450 17A1; cytochrome p450 XVIIA1; cytochrome P450, family 17, subfamily A, polypeptide 1; cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia; cytochrome P450c17; cytochrome P450-C17; P450C17; S17AH; steroid 17-alpha-hydroxylase/17,20 lyase; steroid 17-alpha-monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05093
  • Protein Name
    Steroid 17-alpha-hydroxylase/17,20 lyase
  • Scientific Description
    This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of CYP17A1 using anti-CYP17A1 antibody (A00615-1). CYP17A1 was detected in paraffin-embedded section of human testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-CYP17A1 Antibody (A00615-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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