Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
![CYR61 antibody [HL2144] detects CYR61 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CYR61 stained by CYR61 antibody [HL2144] (GTX638122) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638122/GTX638122_T-44928_20230217_IHC-P_23021401_840.webp "CYR61 antibody [HL2144] detects CYR61 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CYR61 stained by CYR61 antibody [HL2144] (GTX638122) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638122/GTX638122_T-44928_20230217_WB_R_muscle_23022022_971.webp "Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638122/GTX638122_44998_20230331_WB_TPM_watermark_23041023_193.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![CYR61 antibody [HL2144] detects CYR61 protein at secreted protein by immunofluorescent analysis. Sample: C2C12 cells were fixed in ice-cold MeOH for 5 min. Green: CYR61 stained by CYR61 antibody [HL2144] (GTX638122) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638122/GTX638122_44998_20230825_ICC_IF_23090619_104.webp "CYR61 antibody [HL2144] detects CYR61 protein at secreted protein by immunofluorescent analysis. Sample: C2C12 cells were fixed in ice-cold MeOH for 5 min. Green: CYR61 stained by CYR61 antibody [HL2144] (GTX638122) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (GTX638122) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CYR61 antibody [HL2144]
GTX638122
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCCN1
Overview
- SupplierGeneTex
- Product NameCYR61 antibody [HL2144]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2144
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3491
- Target nameCCN1
- Target descriptioncellular communication network factor 1
- Target synonymsCYR61, GIG1, IGFBP10, CCN family member 1, IBP-10, IGF-binding protein 10, IGFBP-10, cysteine rich angiogenic inducer 61, cysteine-rich heparin-binding protein 61, cysteine-rich, anigogenic inducer, 61, insulin-like growth factor-binding protein 10, protein CYR61
- HostRabbit
- IsotypeIgG
- Protein IDO00622
- Protein NameCCN family member 1
- Scientific DescriptionThe secreted protein encoded by this gene is growth factor-inducible and promotes the adhesion of endothelial cells. The encoded protein interacts with several integrins and with heparan sulfate proteoglycan. This protein also plays a role in cell proliferation, differentiation, angiogenesis, apoptosis, and extracellular matrix formation. [provided by RefSeq, Sep 2011]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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