Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638209/GTX638209_T-44942_20230303_IHC-P_23031402_575.webp "Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638209/GTX638209_T-44942_20230331_ICC_IF_23041023_864.webp "Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230421_WB_TPM_watermark_23042500_470.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Whole cell extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230519_WB_R_23053001_233.webp "Whole cell extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230519_WB_R_brain_23053001_262.webp "Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230707_WB_shRNA_watermark_23071300_650.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20230721_IHC-P_Cat_23073119_569.webp "Cytochrome C antibody [HL2205] detects Cytochrome C protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded cat kidney. Cytochrome C stained by Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638209/GTX638209_45012_20241011_WB_24101600_763.webp "Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [HL2205] (GTX638209) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cytochrome C antibody [HL2205]
GTX638209
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFeline, Human, Mouse, Rat
TargetCYCS
Overview
- SupplierGeneTex
- Product NameCytochrome C antibody [HL2205]
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2205
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID54205
- Target nameCYCS
- Target descriptioncytochrome c, somatic
- Target synonymsCYC, HCS, THC4, cytochrome c
- HostRabbit
- IsotypeIgG
- Protein IDP99999
- Protein NameCytochrome c
- Scientific DescriptionThis gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
- ReactivityFeline, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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