![Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110414/GTX110414_40814_20170629_WB_KO_watermark_w_23060500_196.webp)
Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Cytokeratin 7 antibody [N2C2], Internal
GTX110414
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetKRT7
Overview
- SupplierGeneTex
- Product NameCytokeratin 7 antibody [N2C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.78 mg/ml
- ConjugateUnconjugated
- Gene ID3855
- Target nameKRT7
- Target descriptionkeratin 7
- Target synonymsCK7, K2C7, K7, SCL, keratin, type II cytoskeletal 7, CK-7, cytokeratin 7, keratin 7, type II, keratin, 55K type II cytoskeletal, keratin, simple epithelial type I, K7, sarcolectin, type II mesothelial keratin K7, type-II keratin Kb7
- HostRabbit
- IsotypeIgG
- Protein IDP08729
- Protein NameKeratin, type II cytoskeletal 7
- Scientific DescriptionThe protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Casique-Aguirre D, Briseño-Díaz P, García-Gutiérrez P, et al. KRas4B-PDE6δ complex stabilization by small molecules obtained by virtual screening affects Ras signaling in pancreatic cancer. BMC Cancer. 2018,18(1):1299. doi: 10.1186/s12885-018-5142-7Read this paper


![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N2C2], Internal (GTX110414) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110414/GTX110414_40814_20200117_WB_w_23060500_374.webp)






