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ProductsBack/Cytokeratin 7/17 (C46): m-IgG Fc BP-HRP Bundle

Cytokeratin 7/17 (C46): m-IgG Fc BP-HRP Bundle

SC-536563
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetKRT7
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Cytokeratin 7/17 (C46): m-IgG Fc BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3855
  • Target name
    KRT7
  • Target description
    keratin 7
  • Target synonyms
    CK7, K2C7, K7, SCL, keratin, type II cytoskeletal 7, CK-7, cytokeratin 7, keratin 7, type II, keratin, 55K type II cytoskeletal, keratin, simple epithelial type I, K7, sarcolectin, type II mesothelial keratin K7, type-II keratin Kb7
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KRT7 using anti-KRT7 antibody (A02416-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 antigen affinity purified polyclonal antibody (Catalog # A02416-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7 at approximately 51 kDa. The expected band size for KRT7 is at 51 kDa.
Product group Antibodies
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ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetKRT7
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Product group Antibodies
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ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetKRT17
  • SizePrice
Product group Antibodies
Biomatik
KRT7 Polyclonal AntibodyCAC14736
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetKRT7
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Product group Antibodies
RayBiotech
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ApplicationsFlow Cytometry
ReactivityHuman
TargetKRT7
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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CK7 Polyclonal Antibody(bs-1610R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
CK7 Polyclonal AntibodyBS-1610R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetKRT7
  • SizePrice
Product group Antibodies
References
GeneTex
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ReactivityHuman
TargetKRT7
  • SizePrice
Product group Antibodies
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ReactivityHuman
TargetKRT7
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Product group Antibodies
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Cytokeratin 7 antibody10R-C174A
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Product group Antibodies
Nordic-MUbio
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ReactivityHuman
TargetKRT7
  • SizePrice
Immunohistochemistry analysis in human placenta and skeletal muscle tissues using AMAb91530 antibody. Corresponding KRT7 RNA-seq data are presented for the same tissues.
Product group Antibodies
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TargetKRT7
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