More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI4D12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. Positive lysates LY419425 (100ug) and LC419425 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. Positive lysates LY419425 (100ug) and LC419425 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. Positive lysates LY419425 (100ug) and LC419425 (20ug) can be purchased separately from OriGene.

Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI4D12]

CF500216
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetKRT8
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI4D12]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4D12
  • Gene ID3856
  • Target name
    KRT8
  • Target description
    keratin 8
  • Target synonyms
    CARD2, CK-8, CK8, CYK8, K2C8, K8, KO, keratin, type II cytoskeletal 8, cytokeratin-8, keratin 8, type II, type-II keratin Kb8
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP05787
  • Protein Name
    Keratin, type II cytoskeletal 8
  • Scientific Description
    Carrier-free (BSA/glycerol-free) CK8 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of Cytokeratin 8 using anti-Cytokeratin 8 antibody (A01421). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: MCF-7 whole cell lysates, Lane 5: A549 whole cell lysates, Lane 6: HELA whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 8 antigen affinity purified polyclonal antibody (Catalog # A01421) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 8 at approximately 54KD, 56KD. The expected band size for Cytokeratin 8 is at 54KD.
Product group Antibodies
Boster Bio
Anti-Cytokeratin 8/KRT8 Antibody Picoband(r)A01421-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetKRT8
  • SizePrice
Product group Antibodies
RayBiotech
Anti-KRT8 Antibody130-10237
ApplicationsWestern Blot, ELISA
  • SizePrice
Anti-Keratin 8 Antibody
Product group Antibodies
Antibodies.com
Anti-Keratin 8 AntibodyA95180
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Absolute Antibody
Anti-Human Cytokeratin 8 [1E8-C6-B4]Ab02687-1.1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
ReactivityHuman
TargetKRT8
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-Cytokeratin 8 (Ser23) Antibody (YA2635, Rabbit)HY-P82890
TargetKRT8
  • SizePrice
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-KRT8-25ulHPA049866
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
IHC staining of human colon cancer tissue, diluted at 1:200.
Product group Antibodies
Cusabio
KRT8 Monoclonal AntibodyCSB-MA000212
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetKRT8
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
KRT8 / CK8 / Cytokeratin 8 Antibody (Preservative Free)LS-C343077
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetKRT8
  • SizePrice
Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoplasm by immunofluorescent analysis. Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (GTX109489) diluted at 1:500.
Product group Antibodies
GeneTex
Cytokeratin 8 antibody [N1N3]GTX109489
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Human, Mouse, Rat, Zebra Fish
TargetKRT8
  • SizePrice
Formalin-fixed and rat colon tissue labeled with Anti-CK8 Polyclonal Antibody, Unconjugated (bs-1106R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss
Cytokeratin 8 Polyclonal AntibodyBS-1106R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetKRT8
  • SizePrice