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Cytokines Growth Factors

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GTX65633-pro CD137L protein (DDDDK tagged) binding to CD137 transfected cells. HEK 293 cells were transiently transfected with a construct encoding full-length mouse CD137. At 48hrs after transfection, the transfectants were incubated with 0.5 or 5μg/ml GTX65633 CD137L protein (DDDDK tagged) and followed by flow cytometry analysis with anti-DDDDK anibody.
Product group Proteins / Signaling Molecules
GeneTex
Mouse 4-1BBL / CD137L protein, DDDDK tagGTX65633-PRO
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"Pull down assay of GTX65634-pro CD137L protein (DDDDK tagged) by hCD137-Fc. 1. hCD137-Fc + hCD137L-DDDDDK-tag 2. hCD137-Fc + hGITRL-DDDDDK-tag 3. hCD137-Fc + hGITRL-His-tag 4. hGITR-Fc + hCD137L-DDDDDK-tag 5. hGITR-Fc + hGITRL-DDDDDK-tag"
Product group Proteins / Signaling Molecules
GeneTex
Human 4-1BBL / CD137L protein, DDDDK tagGTX65634-PRO
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GTX65635-pro CD40L protein (multimeric) does not need an enhancer to induce B cells activation. Method: PBL cells were incubated in 96-well plates (2x10 5 cells/well in 100μl RPMI supplemented with 10% FCS) for 24 hours at 37oC with the indicated concentration of CD40L (multimeric) or CD40L in the presence and absence of 1μg/ml Enhancer. Cells were washed with PBS and stained with CD86-PE antibody and CD19-FITC antibody, and then analyzed by flow cytometry.
Product group Proteins / Signaling Molecules
GeneTex
Human CD40L / CD154 protein, DDDDK tag (active, multimeric)GTX65635-PRO
ApplicationsFunctional Assay
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Deglycosylation of GTX65638-pro rat progranulin (DDDDK tagged) protein. To examine the deglycosylation of rat Progranulin (DDDDDK tagged), 1 μg of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, twofold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37oC. Separation of reaction products is visualized by immunoblotting using anti-DDDDDK-HRP antibody.
Product group Proteins / Signaling Molecules
GeneTex
Rat Progranulins protein, DDDDK tagGTX65638-PRO
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Deglycosylation of GTX65641-pro Progranulin (human) protein. To examine the deglycosylation of human Progranulin, 1 μg of human progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37oC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (human) antibody.
Product group Proteins / Signaling Molecules
GeneTex
Human Progranulins protein (active)GTX65641-PRO
ApplicationsFunctional Assay
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Deglycosylation of GTX65642-pro Progranulin (mouse) protein.To examine the deglycosylation of mouse Progranulin, 1 μg of mouse progranulin is denatured with 1X glycoprotein denaturing buffer at 100oC for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37oC. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (mouse) antibody.
Product group Proteins / Signaling Molecules
GeneTex
Mouse Progranulins proteinGTX65642-PRO
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Product group Proteins / Signaling Molecules
GeneTex
Mouse IL1 Beta proteinGTX65651-PRO
Protein IDP10749
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Product group Proteins / Signaling Molecules
GeneTex
Mouse APRIL protein, DDDDK tag (active, multimeric)GTX65652-PRO
ApplicationsFunctional Assay
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Product group Proteins / Signaling Molecules
GeneTex
Mouse IL1F6 protein (active)GTX65653-PRO
ApplicationsFunctional Assay
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Product group Proteins / Signaling Molecules
GeneTex
Mouse IL1F8 protein (active)GTX65654-PRO
ApplicationsFunctional Assay
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Product group Proteins / Signaling Molecules
GeneTex
Mouse PEDF protein, DDDDK tagGTX65656-PRO
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Product group Proteins / Signaling Molecules
GeneTex
Mouse CD252 protein, DDDDK tag (active, multimeric)GTX65660-PRO
ApplicationsFunctional Assay
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