DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.
![DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632065/GTX632065_41967_20150515_WB_R_w_23061202_551.webp "DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.")
![DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. DCK antibody [GT10412] (GTX632065) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632065/GTX632065_41967_20150409_IHC_2_w_23061202_811.webp "DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. DCK antibody [GT10412] (GTX632065) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. DCK antibody [GT10412] (GTX632065) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632065/GTX632065_41967_20150409_IHC_w_23061202_418.webp "DCK antibody [GT10412] detects DCK protein at nucleus and cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. DCK antibody [GT10412] (GTX632065) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632065/GTX632065_41967_20160728_WB_w_23061202_779.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:500.")
![Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 μg of DCK antibody [GT10412] (GTX632065) or DCK antibody (GTX102800). Western blot analysis was performed using DCK antibody [GT10412] (GTX632065) diluted at 1:500. EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632065/GTX632065_41967_20170428_IP_w_23061202_585.webp "Immunoprecipitation of DCK protein from HCT-116 whole cell extract using 5 μg of DCK antibody [GT10412] (GTX632065) or DCK antibody (GTX102800). Western blot analysis was performed using DCK antibody [GT10412] (GTX632065) diluted at 1:500. EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632065/GTX632065_41967_20180817_WB_B_w_23061202_944.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.")
![DCK antibody [GT10412] detects DCK protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: DCK protein stained by DCK antibody [GT10412] (GTX632065) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632065/GTX632065_41967_20160301_IFA_w_23061202_410.webp "DCK antibody [GT10412] detects DCK protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: DCK protein stained by DCK antibody [GT10412] (GTX632065) diluted at 1:500. Blue: Hoechst 33342 staining.")
DCK antibody [GT10412] detects DCK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody [GT10412] (GTX632065) diluted at a dilution of 1:500.
DCK antibody [GT10412]
GTX632065
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDCK
Overview
- SupplierGeneTex
- Product NameDCK antibody [GT10412]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT10412
- Concentration2.01 mg/ml
- ConjugateUnconjugated
- Gene ID1633
- Target nameDCK
- Target descriptiondeoxycytidine kinase
- Target synonymsdeoxycytidine kinase, deoxyadenosine kinase, deoxyguanosine kinase, deoxynucleoside kinase
- HostMouse
- IsotypeIgG2b
- Protein IDP27707
- Protein NameDeoxycytidine kinase
- Scientific DescriptionDeoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Cho CJ, Yang CW, Wu CL, et al. The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol. Oncol Lett. 2019,18(6):6869-6876. doi: 10.3892/ol.2019.11023Read this paper
Datasheet
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Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX632062/GTX632062_41946_20180511_IHC-P_R_w_23061202_541.webp)
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