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Western Blot analysis of K562 cells using DDR1 Polyclonal Antibody
Western Blot analysis of K562 cells using DDR1 Polyclonal Antibody
Western Blot analysis of K562 cells using DDR1 Polyclonal Antibody

DDR1 Antibody

CSB-PA002098
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetDDR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DDR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID780
  • Target name
    DDR1
  • Target description
    discoidin domain receptor tyrosine kinase 1
  • Target synonyms
    CAK, CD167, DDR, EDDR1, HGK2, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE, epithelial discoidin domain-containing receptor 1, CD167 antigen-like family member A, PTK3A protein tyrosine kinase 3A, cell adhesion kinase, mammary carcinoma kinase 10, neuroepithelial tyrosine kinase, neurotrophic tyrosine kinase, receptor, type 4, protein-tyrosine kinase RTK-6, tyrosine kinase DDR, tyrosine-protein kinase CAK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08345
  • Protein Name
    Epithelial discoidin domain-containing receptor 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DDR1 using anti-DDR1 antibody (A00905). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR1 antigen affinity purified polyclonal antibody (Catalog # A00905) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR1 at approximately 101KD. The expected band size for DDR1 is at 101KD.
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