Bio-Connect

DDR1 antibody [C1C3]

GTX111312
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDDR1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    DDR1 antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.03 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID780
  • Target name
    DDR1
  • Target description
    discoidin domain receptor tyrosine kinase 1
  • Target synonyms
    CAK; CD167; CD167 antigen-like family member A; cell adhesion kinase; DDR; EDDR1; epithelial discoidin domain-containing receptor 1; HGK2; mammary carcinoma kinase 10; MCK10; NEP; neuroepithelial tyrosine kinase; neurotrophic tyrosine kinase, receptor, type 4; NTRK4; protein-tyrosine kinase RTK-6; PTK3; PTK3A; PTK3A protein tyrosine kinase 3A; RTK6; TRKE; tyrosine kinase DDR; tyrosine-protein kinase CAK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08345
  • Protein Name
    Epithelial discoidin domain-containing receptor 1
  • Scientific Description
    Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation and metabolism. The protein encoded by this gene is a RTK that is widely expressed in normal and transformed epithelial cells and is activated by various types of collagen. This protein belongs to a subfamily of tyrosine kinase receptors with a homology region to the Dictyostelium discoideum protein discoidin I in their extracellular domain. Its autophosphorylation is achieved by all collagens so far tested (type I to type VI). In situ studies and Northern-blot analysis showed that expression of this encoded protein is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, this protein is significantly over-expressed in several human tumors from breast, ovarian, esophageal, and pediatric brain. This gene is located on chromosome 6p21.3 in proximity to several HLA class I genes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetDDR1
  • SizePrice
Product group Antibodies
Abbexa
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Anti-DDR1 Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
  • SizePrice
WB analysis of MCF-7 whole cell lysate using GTX25508 DDR1 antibody. Loading : 20 microg per lane Dilution : 1:2000
Product group Antibodies
References
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetDDR1
  • SizePrice
Figure 1. Western blot analysis of DDR1 using anti-DDR1 antibody (A00905). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR1 antigen affinity purified polyclonal antibody (Catalog # A00905) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR1 at approximately 101KD. The expected band size for DDR1 is at 101KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetDDR1
  • SizePrice
Product group Antibodies
Assay Genie
TargetDDR1
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetDDR1
  • SizePrice
Western Blot analysis of K562 cells using DDR1 Polyclonal Antibody
Product group Antibodies
Cusabio
DDR1 AntibodyCSB-PA002098
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetDDR1
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsWestern Blot, ELISA, Neutralisation/Blocking
TargetDDR1
  • SizePrice
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti MCK10/CD167a/DDR1 Polyclonal Antibody, Unconjugated (bs-0671R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetDDR1
  • SizePrice