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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti MCK10/CD167a/DDR1 Polyclonal Antibody, Unconjugated (bs-0671R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti MCK10/CD167a/DDR1 Polyclonal Antibody, Unconjugated (bs-0671R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti MCK10/CD167a/DDR1 Polyclonal Antibody, Unconjugated (bs-0671R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

DDR1 Polyclonal Antibody

BS-0671R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetDDR1
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Overview

  • Supplier
    Bioss
  • Product Name
    MCK10 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID780
  • Target name
    DDR1
  • Target description
    discoidin domain receptor tyrosine kinase 1
  • Target synonyms
    CAK, CD167, DDR, EDDR1, HGK2, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE, epithelial discoidin domain-containing receptor 1, CD167 antigen-like family member A, PTK3A protein tyrosine kinase 3A, cell adhesion kinase, mammary carcinoma kinase 10, neuroepithelial tyrosine kinase, neurotrophic tyrosine kinase, receptor, type 4, protein-tyrosine kinase RTK-6, tyrosine kinase DDR, tyrosine-protein kinase CAK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08345
  • Protein Name
    Epithelial discoidin domain-containing receptor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Activated hair follicle stem cells and Wnt/beta-catenin signaling involve in pathnogenesis of sebaceous neoplasms. Qiu W et al., 2014, Int J Med Sci
    Read more

Datasheet

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Figure 1. Western blot analysis of DDR1 using anti-DDR1 antibody (A00905). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR1 antigen affinity purified polyclonal antibody (Catalog # A00905) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDR1 at approximately 101KD. The expected band size for DDR1 is at 101KD.
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