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ProductsBack/DDX58 Antibody (YA2318, Rabbit)

DDX58 Antibody (YA2318, Rabbit)

HY-P82573
MedChem Express
TargetDDX58
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    DDX58 Antibody (YA2318, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID23586
  • Target name
    DDX58
  • Target description
    DExD/H-box helicase 58
  • Target synonyms
    antiviral innate immune response receptor RIG-I; DEAD (Asp-Glu-Ala-Asp) box polypeptide 58; DEAD box protein 58; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide; probable ATP-dependent RNA helicase DDX58; retinoic acid-inducible gene 1 protein; retinoic acid-inducible gene I protein; RIG1; RIGI; RIG-I; RLR-1; RNA helicase RIG-I; SGMRT2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    DDX58 Antibody (YA2318) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2318), targeting DDX58, with a predicted molecular weight of 107 kDa (observed band size: 107 kDa). DDX58 Antibody (YA2318) can be used for WB, IP experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of DDX58 using anti-DDX58 antibody (A00244-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat C6 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX58 antigen affinity purified polyclonal antibody (Catalog # A00244-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX58 at approximately 107 kDa. The expected band size for DDX58 is at 107 kDa.
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