IHC-P analysis of human colon carcinoma tissue using GTX15827 Diablo antibody [SMAC 17 1-87]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
![IHC-P analysis of human testis tissue using GTX15827 Diablo antibody [SMAC 17 1-87]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX15827/GTX15827_1047_IHC-P_w_23060620_440.webp "IHC-P analysis of human testis tissue using GTX15827 Diablo antibody [SMAC 17 1-87]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100")
![IHC-P analysis of mouse testis tissue using GTX15827 Diablo antibody [SMAC 17 1-87]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX15827/GTX15827_1048_IHC-P_w_23060620_926.webp "IHC-P analysis of mouse testis tissue using GTX15827 Diablo antibody [SMAC 17 1-87]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100")
![WB analysis of 25 ug of 293 (Lane 1), Hela (Lane 2), and PC12 cell lysates (Lane 3) using GTX15827 Diablo antibody [SMAC 17 1-87]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX15827/GTX15827_1545_WB_w_23060620_654.webp "WB analysis of 25 ug of 293 (Lane 1), Hela (Lane 2), and PC12 cell lysates (Lane 3) using GTX15827 Diablo antibody [SMAC 17 1-87]. Dilution : 1:1000")
![ICC/IF analysis of MCF-7 cells using GTX15827 Diablo antibody [SMAC 17 1-87]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15827/GTX15827_327_ICC-IF_w_23060620_640.webp "ICC/IF analysis of MCF-7 cells using GTX15827 Diablo antibody [SMAC 17 1-87]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC")
![ICC/IF analysis of PC-12 cells using GTX15827 Diablo antibody [SMAC 17 1-87]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15827/GTX15827_328_ICC-IF_w_23060620_299.webp "ICC/IF analysis of PC-12 cells using GTX15827 Diablo antibody [SMAC 17 1-87]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC")
![ICC/IF analysis of A431 cells using GTX15827 Diablo antibody [SMAC 17 1-87]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15827/GTX15827_329_ICC-IF_w_23060620_598.webp "ICC/IF analysis of A431 cells using GTX15827 Diablo antibody [SMAC 17 1-87]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight at 4 oC")
IHC-P analysis of human colon carcinoma tissue using GTX15827 Diablo antibody [SMAC 17 1-87]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
Diablo antibody [SMAC 17 1-87]
GTX15827
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDIABLO
Overview
- SupplierGeneTex
- Product NameDiablo antibody [SMAC 17 1-87]
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:20-1:200. IHC-P: 1:20-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSMAC 17 1-87
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID56616
- Target nameDIABLO
- Target descriptiondiablo IAP-binding mitochondrial protein
- Target synonymsDFNA64; diablo homolog, mitochondrial; diablo-like protein; direct IAP-binding protein with low pI; second mitochondria-derived activator of caspase; SMAC
- HostMouse
- IsotypeIgG1
- Protein IDQ9NR28
- Protein NameDiablo homolog, mitochondrial
- Scientific DescriptionThis gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203