Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dicer stained by Dicer antibody [HL1232] (GTX636580) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636580/GTX636580_T-44494_20220826_ICC_IF_22083119_510.webp "Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dicer stained by Dicer antibody [HL1232] (GTX636580) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636580/GTX636580_44536_20221209_WB_D_C_22121123_109.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636580/GTX636580_44536_20211231_WB_w_23061202_729.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Dicer stained by Dicer antibody [HL1232] (GTX636580) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636580/GTX636580_T-44494_20220114_IHC-P_M_w_23061202_859.webp "Dicer antibody [HL1232] detects Dicer protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Dicer stained by Dicer antibody [HL1232] (GTX636580) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636580/GTX636580_T-44494_20211126_WB_R_w_23061202_994.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636580/GTX636580_T-44494_20211126_WB_M_w_23061202_417.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody [HL1232] (GTX636580) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Dicer antibody [HL1232]
GTX636580
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Feline, Human, Mouse, Rat
TargetDICER1
Overview
- SupplierGeneTex
- Product NameDicer antibody [HL1232]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1232
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID23405
- Target nameDICER1
- Target descriptiondicer 1, ribonuclease III
- Target synonymsDCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD, endoribonuclease Dicer, Dicer1, Dcr-1 homolog, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, helicase MOI, helicase with RNAse motif
- HostRabbit
- IsotypeIgG
- Protein IDQ9UPY3
- Protein NameEndoribonuclease Dicer
- Scientific DescriptionThis gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
- ReactivityCanine, Feline, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![Dicer antibody [GT1176] detects Dicer protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dicer stained by Dicer antibody [GT1176] (GTX633917) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX633917/GTX633917_42814_20180321_ICC_IF_w_23061202_996.webp)
Product group Antibodies
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ReactivityHuman
TargetDICER1
- SizePrice