More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/DICER1 Antibody
IHC image of CSB-PA891575LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA891575LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA891575LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

DICER1 Antibody

CSB-PA891575LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetDICER1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    DICER1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23405
  • Target name
    DICER1
  • Target description
    dicer 1, ribonuclease III
  • Target synonyms
    DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD, endoribonuclease Dicer, Dicer1, Dcr-1 homolog, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, helicase MOI, helicase with RNAse motif
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UPY3
  • Protein Name
    Endoribonuclease Dicer
  • Scientific Description
    Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3 overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biomatik
Dicer 1, Ribonuclease Type III (DICER1) Polyclonal AntibodyCAU21566
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetDICER1
  • SizePrice
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA447308(DICER1 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
Product group Antibodies
Cusabio
DICER1 AntibodyCSB-PA447308
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetDICER1
  • SizePrice
Figure 1. Western blot analysis of Dicer/DICER1 using anti-Dicer/DICER1 antibody (A00190-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dicer/DICER1 antigen affinity purified polyclonal antibody (Catalog # A00190-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dicer/DICER1 at approximately 220-250 kDa. The expected band size for Dicer/DICER1 is at 219 kDa.
Product group Antibodies
Boster Bio
Anti-Dicer/DICER1 Antibody Picoband(r)A00190-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetDICER1
  • SizePrice
Immunohistochemical staining of human uterus shows cytoplasmic immunoreactivity in the glandular epithelium cells.
Product group Antibodies
Atlas Antibodies
Anti-DICER1 AntibodyAMAB90737
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetDICER1
  • SizePrice
Anti-Dicer Antibody
Product group Antibodies
Antibodies.com
Anti-Dicer AntibodyA326239
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
RayBiotech
Anti-DICER1 Antibody144-06021
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetDICER1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-Dicer1EB06871
ApplicationsELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetDICER1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
DICER1 / Dicer AntibodyLS-C403112
ApplicationsELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetDICER1
  • SizePrice
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (DICER1) polyclonal Antibody, Unconjugated (bs-6697R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Product group Antibodies
Bioss
DICER1 Polyclonal AntibodyBS-6697R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Human, Mouse, Porcine, Rat
TargetDICER1
  • SizePrice
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Dicer antibody (GTX130536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
Dicer antibodyGTX130536
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetDICER1
  • SizePrice