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Western blot All lanes: DNAJB1 antibody at 3microg/ml Lane 1: Mouse spleen tissue Lane 2: Colo320 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: LO2 whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: HT29 whole cell lysate Lane 7: A549 whole cell lysate Lane 8: Jurkat whole cell lysate Lane 9: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 28 kDa Observed band size: 39 kDa
Western blot All lanes: DNAJB1 antibody at 3microg/ml Lane 1: Mouse spleen tissue Lane 2: Colo320 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: LO2 whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: HT29 whole cell lysate Lane 7: A549 whole cell lysate Lane 8: Jurkat whole cell lysate Lane 9: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 28 kDa Observed band size: 39 kDa
Western blot All lanes: DNAJB1 antibody at 3microg/ml Lane 1: Mouse spleen tissue Lane 2: Colo320 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: LO2 whole cell lysate Lane 5: HepG2 whole cell lysate Lane 6: HT29 whole cell lysate Lane 7: A549 whole cell lysate Lane 8: Jurkat whole cell lysate Lane 9: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 39, 28 kDa Observed band size: 39 kDa

DNAJB1 Antibody

CSB-PA03934A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetDNAJB1
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Overview

  • Supplier
    Cusabio
  • Product Name
    DNAJB1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3337
  • Target name
    DNAJB1
  • Target description
    DnaJ heat shock protein family (Hsp40) member B1
  • Target synonyms
    HSPF1, Hdj1, Hsp40, RSPH16B, Sis1, dnaJ homolog subfamily B member 1, DnaJ (Hsp40) homolog, subfamily B, member 1, dnaJ protein homolog 1, heat shock 40 kDa protein 1, human DnaJ protein 1, radial spoke 16 homolog B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25685
  • Protein Name
    DnaJ homolog subfamily B member 1
  • Scientific Description
    Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. Negatively regulates heat shock-induced HSF1 transcriptional activity during the attenuation and recovery phase period of the heat shock response (PubMed:9499401). Stimulates ATP hydrolysis and the folding of unfolded proteins mediated by HSPA1A/B (in vitro) (PubMed:24318877).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: 293T cell lysates; Lane 2: MCF-7 lysates; Lane 3: BxPC-3 cell lysates; Lane 4: BT-474 cell lysates; Lane 5: SW620 cell lysates; Lane 6: K562 cell lysates; Lane 7: Mouse testis lysates; Lane 8: Mouse spleen lysates; Lane 9: Mouse thymus lysates probed with DNAJB1 Polyclonal Antibody, Unconjugated (bs-55055R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Hsp40/DNAJB1 using anti-Hsp40/DNAJB1 antibody (A03100-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp40/DNAJB1 antigen affinity purified polyclonal antibody (Catalog # A03100-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp40/DNAJB1 at approximately 38 kDa. The expected band size for Hsp40/DNAJB1 is at 38 kDa.
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