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ProductsBack/DUSP1 (phospho Ser296/Ser318) antibody
Western blot analysis of MKP-1/2 phosphorylation expression in heatshockK562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of MKP-1/2 phosphorylation expression in heatshockK562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of MKP-1/2 phosphorylation expression in heatshockK562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

DUSP1 (phospho Ser296/Ser318) antibody

GTX52320
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetDUSP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    DUSP1 (phospho Ser296/Ser318) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB 1:500-1:2000, IHC 1:50-1:200
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1843
  • Target name
    DUSP1
  • Target description
    dual specificity phosphatase 1
  • Target synonyms
    CL100, HVH1, MKP-1, MKP1, PTPN10, dual specificity protein phosphatase 1, CL 100, MAP kinase phosphatase 1, dual specificity protein phosphatase hVH1, mitogen-activated protein kinase phosphatase 1, protein-tyrosine phosphatase CL100, serine/threonine specific protein phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28562
  • Protein Name
    Dual specificity protein phosphatase 1
  • Scientific Description
    The protein encoded by this gene is a phosphatase with dual specificity for tyrosine and threonine. The encoded protein can dephosphorylate MAP kinase MAPK1/ERK2, which results in its involvement in several cellular processes. This protein appears to play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. Finally, the encoded protein can make some solid tumors resistant to both chemotherapy and radiotherapy, making it a target for cancer therapy. [provided by RefSeq, Aug 2017]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MKP-1/DUSP1 using anti-MKP-1/DUSP1 antibody (A02276-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MKP-1/DUSP1 antigen affinity purified polyclonal antibody (Catalog # A02276-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MKP-1/DUSP1 at approximately 40KD. The expected band size for MKP-1/DUSP1 is at 40KD.
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Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with DUSP1/MKP1 Polyclonal Antibody, Unconjugated (bs-1851R) at 1:400 overnight at 4°C, DAB staining.
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