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ProductsBack/Dystrophin antibody [MANDYS8]
IHC-Fr analysis of acetone-fixed human tongue tissue using GTX27163 Dystrophin antibody [MANDYS8] at 1:400.
IHC-Fr analysis of acetone-fixed human tongue tissue using GTX27163 Dystrophin antibody [MANDYS8] at 1:400.
IHC-Fr analysis of acetone-fixed human tongue tissue using GTX27163 Dystrophin antibody [MANDYS8] at 1:400.

Dystrophin antibody [MANDYS8]

GTX27163
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
DownloadDatasheet
Product group Antibodies
TargetDMD
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Overview

  • Supplier
    GeneTex
  • Product Name
    Dystrophin antibody [MANDYS8]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-Fr: 1:400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MANDYS8
  • Conjugate
    Unconjugated
  • Gene ID1756
  • Target name
    DMD
  • Target description
    dystrophin
  • Target synonyms
    BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; dystrophin; MRX85; truncated dystrophin
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP11532
  • Protein Name
    Dystrophin
  • Scientific Description
    The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • A dystrophic Duchenne mouse model for testing human antisense oligonucleotides. Veltrop M et al., 2018, PLoS One
    Read more
  • Correction of diverse muscular dystrophy mutations in human engineered heart muscle by single-site genome editing.
    Read more

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Figure 1. Western blot analysis of Dystrophin using anti-Dystrophin antibody (PB9276). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse HEPA1-6 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dystrophin antigen affinity purified polyclonal antibody (Catalog # PB9276) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dystrophin at approximately 427KD. The expected band size for Dystrophin is at 427KD.
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