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Dystrophin Antibody (YA3224, Rabbit)

HY-P83479
MedChem Express
TargetDMD
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Dystrophin Antibody (YA3224, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID1756
  • Target name
    DMD
  • Target description
    dystrophin
  • Target synonyms
    BMD, CMD3B, DXS142, DXS164, DXS206, DXS230, DXS239, DXS268, DXS269, DXS270, DXS272, MRX85, dystrophin, mutant dystrophin
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Dystrophin Antibody (YA3224) is a non-conjugated IgG antibody, targeting Dystrophin, with a predicted molecular weight of 427 kDa (observed band size: 427 kDa). Dystrophin Antibody (YA3224) can be used for WB experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Dystrophin using anti-Dystrophin antibody (PB9276). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse HEPA1-6 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dystrophin antigen affinity purified polyclonal antibody (Catalog # PB9276) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dystrophin at approximately 427KD. The expected band size for Dystrophin is at 427KD.
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