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ECE-1 Antibody

LS-C400720
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetECE1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ECE-1 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.9 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1889
  • Target name
    ECE1
  • Target description
    endothelin converting enzyme 1
  • Target synonyms
    ECE, endothelin-converting enzyme 1, ECE-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ECE1 using anti-ECE1 antibody (A02519-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECE1 antigen affinity purified polyclonal antibody (Catalog # A02519-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECE1 at approximately 87KD. The expected band size for ECE1 is at 87KD.
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