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Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA007371ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA007371ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA007371ESR2HU at dilution of 1:100

ECE1 Antibody

CSB-PA007371ESR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetECE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ECE1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1889
  • Target name
    ECE1
  • Target description
    endothelin converting enzyme 1
  • Target synonyms
    ECE, endothelin-converting enzyme 1, ECE-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42892
  • Protein Name
    Endothelin-converting enzyme 1
  • Scientific Description
    Converts big endothelin-1 to endothelin-1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ECE1 using anti-ECE1 antibody (A02519-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECE1 antigen affinity purified polyclonal antibody (Catalog # A02519-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECE1 at approximately 87KD. The expected band size for ECE1 is at 87KD.
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