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ProductsBack/ECE1 Polyclonal Antibody
Formalin-fixed and rat intestine tissue labeled with Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated (bs-1190R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and rat intestine tissue labeled with Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated (bs-1190R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and rat intestine tissue labeled with Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated (bs-1190R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

ECE1 Polyclonal Antibody

BS-1190R
Bioss Antibodies
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetECE1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    ECE1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1889
  • Target name
    ECE1
  • Target description
    endothelin converting enzyme 1
  • Target synonyms
    ECE; ECE-1; endothelin-converting enzyme 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42892
  • Protein Name
    Endothelin-converting enzyme 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ECE1 using anti-ECE1 antibody (A02519-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse heart tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ECE1 antigen affinity purified polyclonal antibody (Catalog # A02519-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ECE1 at approximately 87KD. The expected band size for ECE1 is at 87KD.
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