IHC-P analysis of human prostate tissue using GTX84582 eIF2 alpha antibody [1H1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
![WB analysis of various cell lines using GTX84582 eIF2 alpha antibody [1H1]. Loading : 35 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84582/GTX84582_3546_WB_w_23061420_874.webp "WB analysis of various cell lines using GTX84582 eIF2 alpha antibody [1H1]. Loading : 35 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with eIF2 alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84582 eIF2 alpha antibody [1H1]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84582/GTX84582_4479_WB_w_23061420_322.webp "WB analysis of HEK293T cells transfected with eIF2 alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84582 eIF2 alpha antibody [1H1]. Loading : 5 ug per lane")
![FACS analysis of Jurkat cells using GTX84582 eIF2 alpha antibody [1H1]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84582/GTX84582_465_FACS_w_23061420_815.webp "FACS analysis of Jurkat cells using GTX84582 eIF2 alpha antibody [1H1]. Red : Primary antibody Blue : Negative control antibody")
IHC-P analysis of human prostate tissue using GTX84582 eIF2 alpha antibody [1H1]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.
eIF2 alpha antibody [1H1]
GTX84582
Overview
- SupplierGeneTex
- Product NameeIF2 alpha antibody [1H1] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. IHC-P: 1:150. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- Clone ID1H1
- Concentration1 mg/ml
- FormulationLiquid
- Gene ID1965
- Target nameEIF2S1
- Target descriptioneukaryotic translation initiation factor 2 subunit alpha
- Target synonymsEIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
- Protein IDP05198
- Protein NameEukaryotic translation initiation factor 2 subunit 1
- Scientific DescriptionFunctions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203