WB analysis of HEK293T cells transfected with eIF2 alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84578 eIF2 alpha antibody [3H7]. Loading : 5 ug per lane
![WB analysis of various cell lines using GTX84578 eIF2 alpha antibody [3H7]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84578/GTX84578_4476_WB_w_23061420_374.webp "WB analysis of various cell lines using GTX84578 eIF2 alpha antibody [3H7]. Loading : 35 ug per lane")
![FACS analysis of Jurkat cells using GTX84578 eIF2 alpha antibody [3H7]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX84578/GTX84578_461_FACS_w_23061420_161.webp "FACS analysis of Jurkat cells using GTX84578 eIF2 alpha antibody [3H7]. Red : Primary antibody Blue : Negative control antibody")
![FACS analysis of HEK293T cells transfected with either eIF2 alpha plasmid(Red) or empty vector control plasmid(Blue) using GTX84578 eIF2 alpha antibody [3H7].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84578/GTX84578_462_FACS_w_23061420_985.webp "FACS analysis of HEK293T cells transfected with either eIF2 alpha plasmid(Red) or empty vector control plasmid(Blue) using GTX84578 eIF2 alpha antibody [3H7].")
WB analysis of HEK293T cells transfected with eIF2 alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84578 eIF2 alpha antibody [3H7]. Loading : 5 ug per lane
eIF2 alpha antibody [3H7]
GTX84578
Overview
- SupplierGeneTex
- Product NameeIF2 alpha antibody [3H7] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- Clone ID3H7
- Concentration0.62 mg/ml
- FormulationLiquid
- Gene ID1965
- Target nameEIF2S1
- Target descriptioneukaryotic translation initiation factor 2 subunit alpha
- Target synonymsEIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
- Protein IDP05198
- Protein NameEukaryotic translation initiation factor 2 subunit 1
- Scientific DescriptionFunctions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203