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ProductsBack/eIF2 alpha antibody [3H7]
WB analysis of HEK293T cells transfected with eIF2 alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84578 eIF2 alpha antibody [3H7]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with eIF2 alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84578 eIF2 alpha antibody [3H7]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with eIF2 alpha plasmid (Right) or empty vector (Left) for 48 hrs using GTX84578 eIF2 alpha antibody [3H7]. Loading : 5 ug per lane

eIF2 alpha antibody [3H7]

GTX84578
GeneTex
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetEIF2S1
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Overview

  • Supplier
    GeneTex
  • Product Name
    eIF2 alpha antibody [3H7] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3H7
  • Concentration
    0.62 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1965
  • Target name
    EIF2S1
  • Target description
    eukaryotic translation initiation factor 2 subunit alpha
  • Target synonyms
    EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit 1; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05198
  • Protein Name
    Eukaryotic translation initiation factor 2 subunit 1
  • Scientific Description
    Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EIF2S1 using anti-EIF2S1 antibody (P04387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S1 antigen affinity purified monoclonal antibody (Catalog # P04387) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S1 at approximately 36 kDa. The expected band size for EIF2S1 is at 36 kDa.
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