More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/eIF2 alpha antibody
WB analysis of various sample lysates using GTX64363 eIF2 alpha antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX64363 eIF2 alpha antibody. Dilution : 1:1000 Loading : 25μg per lane
WB analysis of various sample lysates using GTX64363 eIF2 alpha antibody. Dilution : 1:1000 Loading : 25μg per lane

eIF2 alpha antibody

GTX64363
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEIF2S1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    eIF2 alpha antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1965
  • Target name
    EIF2S1
  • Target description
    eukaryotic translation initiation factor 2 subunit alpha
  • Target synonyms
    EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, eukaryotic translation initiation factor 2 subunit 1, eIF-2-alpha, eIF2-alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05198
  • Protein Name
    Eukaryotic translation initiation factor 2 subunit 1
  • Scientific Description
    The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
eIF2alpha AntibodyCAB0764
ReactivityHuman
TargetEIF2S1
  • SizePrice
Western Blot analysis of 3T3 cells using eIF2alpha Polyclonal Antibody
Product group Antibodies
Cusabio
EIF2S1 AntibodyCSB-PA002295
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetEIF2S1
  • SizePrice
Anti-eIF2 alpha Antibody
Product group Antibodies
Antibodies.com
Anti-eIF2 alpha AntibodyA95082
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-EIF2S1 (Ser51) Antibody (YA203, Rabbit)HY-P80811
TargetEIF2S1
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Product group Antibodies
Atlas Antibodies
Anti-EIF2S1 AntibodyHPA064885
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetEIF2S1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
EIF2S1 AntibodyLS-C331101
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetEIF2S1
  • SizePrice
Rabbit anti EIF2alpha [pS51] phosphospecific polyclonal antibody. Left: Image shows immunocytochemical analysis of EIF2alpha [pS51] in rat brain (mediodorsal neocortex) sections following traumatic axonal injury (TAI) (24 h). Control (prior to injury induction) shows low basal levels of EIF2alpha [pS51]. Right: Image shows double labeling of APP (red) and EIF2alpha [pS51] (green) in neurons subjected to traumatic axonal injury (TAI). Inset (above): higher magnification of neuronal soma with TAI. EIF2alpha [pS51] immunofluorescence is localized in cytoplasm of neuronal cell body.
Product group Antibodies
References
GeneTex
eIF2 alpha (phospho Ser51) antibodyGTX24837
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityC. Elegans, Chicken, Drosophila, Feline, Human, Mouse, Primate, Rat, Yeast
TargetEIF2S1
  • SizePrice
Product group Antibodies
Biomatik
Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1) Polyclonal AntibodyCAU31778
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetEIF2S1
  • SizePrice
Figure 1. Western blot analysis of EIF2S1 using anti-EIF2S1 antibody (P04387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S1 antigen affinity purified monoclonal antibody (Catalog # P04387) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S1 at approximately 36 kDa. The expected band size for EIF2S1 is at 36 kDa.
Product group Antibodies
References
Boster Bio
Anti-Phospho-EIF2S1 (S51) Rabbit Monoclonal AntibodyP04387
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetEIF2S1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-eIF2 alpha Polyclonal Antibody, Unconjugated (bs-3613R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Product group Antibodies
References
Bioss
EIF2S1 Polyclonal AntibodyBS-3613R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Chicken, Human, Mouse, Rabbit, Rat
TargetEIF2S1
  • SizePrice