ELISA analysis of antigen using GTX60420 eIF4E antibody [5D11]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
![FACS analysis of HeLa cells using GTX60420 eIF4E antibody [5D11]. Green : eIF4E Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_FACS_w_23061123_405.webp "FACS analysis of HeLa cells using GTX60420 eIF4E antibody [5D11]. Green : eIF4E Purple : negative control")
![ICC/IF analysis of GC-7901 cells using GTX60420 eIF4E antibody [5D11]. Green : eIF4E Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_ICCIF_w_23061123_652.webp "ICC/IF analysis of GC-7901 cells using GTX60420 eIF4E antibody [5D11]. Green : eIF4E Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments")
![IHC-P analysis of stomach cancer (left) and prostate cancer (right) using GTX60420 eIF4E antibody [5D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_IHC-P_1_w_23061123_518.webp "IHC-P analysis of stomach cancer (left) and prostate cancer (right) using GTX60420 eIF4E antibody [5D11].")
![IHC-P analysis of liver cancer (left) and submaxillary tumor (right) using GTX60420 eIF4E antibody [5D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_IHC-P_w_23061123_620.webp "IHC-P analysis of liver cancer (left) and submaxillary tumor (right) using GTX60420 eIF4E antibody [5D11].")
![WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60420/GTX60420_20170912_WB_w_23061123_620.webp "WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].")
ELISA analysis of antigen using GTX60420 eIF4E antibody [5D11]. Red : Control antigen 100ng Purple : Antigen 10ng Green : Antigen 50ng Blue : Antigen 100ng
eIF4E antibody [5D11]
GTX60420
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetEIF4E
Overview
- SupplierGeneTex
- Product NameeIF4E antibody [5D11]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5D11
- ConjugateUnconjugated
- Gene ID1977
- Target nameEIF4E
- Target descriptioneukaryotic translation initiation factor 4E
- Target synonymsAUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein
- HostMouse
- IsotypeIgG1
- Protein IDP06730
- Protein NameEukaryotic translation initiation factor 4E
- Scientific DescriptionThe protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5 end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203