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WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].
WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].
WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].

eIF4E antibody [5D11]

GTX60420
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetEIF4E
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Overview

  • Supplier
    GeneTex
  • Product Name
    eIF4E antibody [5D11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5D11
  • Conjugate
    Unconjugated
  • Gene ID1977
  • Target name
    EIF4E
  • Target description
    eukaryotic translation initiation factor 4E
  • Target synonyms
    AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E, eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, eukaryotic translation initiation factor 4E-like 1, mRNA cap-binding protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP06730
  • Protein Name
    Eukaryotic translation initiation factor 4E
  • Scientific Description
    The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5 end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EIF4E using anti-EIF4E antibody (M00135). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse HEPA1-2 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4E antigen affinity purified monoclonal antibody (Catalog # M00135) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4E at approximately 25 kDa. The expected band size for EIF4E is at 25 kDa.
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FACS analysis of HL-60 cells using GTX82525 eIF4E antibody. Top histogram : negative control Bottom histogram : HL-60 cells
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