More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/eIF4E antibody
FACS analysis of HL-60 cells using GTX82525 eIF4E antibody. Top histogram : negative control Bottom histogram : HL-60 cells
FACS analysis of HL-60 cells using GTX82525 eIF4E antibody. Top histogram : negative control Bottom histogram : HL-60 cells
FACS analysis of HL-60 cells using GTX82525 eIF4E antibody. Top histogram : negative control Bottom histogram : HL-60 cells

eIF4E antibody

GTX82525
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mammals
TargetEIF4E
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    eIF4E antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. IHC-P: 1:50-1:100. FACS: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1977
  • Target name
    EIF4E
  • Target description
    eukaryotic translation initiation factor 4E
  • Target synonyms
    AUTS19, CBP, EIF4E1, EIF4EL1, EIF4F, eIF-4E, eukaryotic translation initiation factor 4E, eIF-4F 25 kDa subunit, eukaryotic translation initiation factor 4E-like 1, mRNA cap-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06730
  • Protein Name
    Eukaryotic translation initiation factor 4E
  • Scientific Description
    The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5 end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  • Reactivity
    Human, Mammals
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Logan SM, Wu CW, Storey KB. The squirrel with the lagging eIF2: Global suppression of protein synthesis during torpor. Comp Biochem Physiol A Mol Integr Physiol. 2019,227:161-171. doi: 10.1016/j.cbpa.2018.10.014
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Abbexa
EIF4E Antibody (HRP)ABX314116
ApplicationsELISA
  • SizePrice
Product group Antibodies
RayBiotech
Anti-EIF4E Antibody144-60024
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman
TargetEIF4E
  • SizePrice
Figure 1. Western blot analysis of EIF4E using anti-EIF4E antibody (M00135). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse HEPA1-2 whole cell lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF4E antigen affinity purified monoclonal antibody (Catalog # M00135) at 1:100 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF4E at approximately 25 kDa. The expected band size for EIF4E is at 25 kDa.
Product group Antibodies
Boster Bio
Anti-EIF4E Monoclonal AntibodyM00135
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetEIF4E
  • SizePrice
eIF4E antibody detects eIF4E protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF4E protein stained by eIF4E antibody (GTX132092) diluted at 1:500. Blue: Hoechst 33342 staining.
Product group Antibodies
References
GeneTex
eIF4E antibodyGTX132092
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetEIF4E
  • SizePrice
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with eIF4E (phospho Ser209) antibody (GTX133606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
eIF4E (phospho Ser209) antibodyGTX133606
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetEIF4E
  • SizePrice
WB analysis of HeLa (1), HEK293 (2) and K562 (3) cell lysate using GTX60420 eIF4E antibody [5D11].
Product group Antibodies
GeneTex
eIF4E antibody [5D11]GTX60420
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetEIF4E
  • SizePrice
eIF4E antibody [HL1553] detects eIF4E protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF4E stained by eIF4E antibody [HL1553] (GTX637028) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
Product group Antibodies
GeneTex
eIF4E antibody [HL1553]GTX637028
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetEIF4E
  • SizePrice
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1554] (GTX637029) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
eIF4E antibody [HL1554]GTX637029
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetEIF4E
  • SizePrice
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody [HL1555] (GTX637030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
eIF4E antibody [HL1555]GTX637030
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetEIF4E
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-EIF4EY058779
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice