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ProductsBack/ELAVL1 Mouse Monoclonal Antibody [Clone ID: OTI14F4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ELAVL1 (Cat# RC201562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELAVL1(Cat# TA500887). Positive lysates [LY400549] (100ug) and [LC400549] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ELAVL1 (Cat# RC201562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELAVL1(Cat# TA500887). Positive lysates [LY400549] (100ug) and [LC400549] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ELAVL1 (Cat# RC201562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELAVL1(Cat# TA500887). Positive lysates [LY400549] (100ug) and [LC400549] (20ug) can be purchased separately from OriGene.

ELAVL1 Mouse Monoclonal Antibody [Clone ID: OTI14F4]

CF500887
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetELAVL1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    ELAVL1 Mouse Monoclonal Antibody [Clone ID: OTI14F4]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI14F4
  • Gene ID1994
  • Target name
    ELAVL1
  • Target description
    ELAV like RNA binding protein 1
  • Target synonyms
    ELAV1, HUR, Hua, MelG, ELAV-like protein 1, ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R), Hu antigen R, Human antigen R, embryonic lethal, abnormal vision, drosophila, homolog-like 1, hu-antigen R
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ15717
  • Protein Name
    ELAV-like protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ELAVL1 mouse monoclonal antibody, clone OTI14F4 (formerly 14F4)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of ELAVL1 using anti-ELAVL1 antibody (M00736). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat spleen tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELAVL1 antigen affinity purified monoclonal antibody (Catalog # M00736) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ELAVL1 at approximately 36 kDa. The expected band size for ELAVL1 is at 36 kDa.
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