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Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A431 whole cell lysate All lanes: ENO1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48, 37 kDa Observed band size: 48 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A431 whole cell lysate All lanes: ENO1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48, 37 kDa Observed band size: 48 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A431 whole cell lysate All lanes: ENO1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48, 37 kDa Observed band size: 48 kDa

ENO1 Antibody

CSB-PA02395A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetENO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ENO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2023
  • Target name
    ENO1
  • Target description
    enolase 1
  • Target synonyms
    ENO1-IT1, ENO1L1, HEL-S-17, MPB1, NNE, PPH, alpha-enolase, c-myc promoter-binding protein-1, 2-phospho-D-glycerate hydro-lyase, ENO1 intronic transcript 1, ENO1 intronic transcript 1 (non-protein coding), MYC promoter-binding protein 1, alpha enolase like 1, enolase 1, (alpha), enolase-alpha, epididymis secretory protein Li 17, non-neural enolase, phosphopyruvate hydratase, plasminogen-binding protein, tau-crystallin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06733
  • Protein Name
    Alpha-enolase
  • Scientific Description
    ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: monkey kidney tissue lysates, Lane 8: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (Catalog # A01250-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENO1 at approximately 47KD. The expected band size for ENO1 is at 47KD.
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