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ENO1 Recombinant Antibody

BSM-61116R
Bioss
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetENO1
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Overview

  • Supplier
    Bioss
  • Product Name
    ENO1 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), IF(ICC)(1:50-200), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2023
  • Target name
    ENO1
  • Target description
    enolase 1
  • Target synonyms
    ENO1-IT1, ENO1L1, HEL-S-17, MPB1, NNE, PPH, alpha-enolase, c-myc promoter-binding protein-1, 2-phospho-D-glycerate hydro-lyase, ENO1 intronic transcript 1, ENO1 intronic transcript 1 (non-protein coding), MYC promoter-binding protein 1, alpha enolase like 1, enolase 1, (alpha), enolase-alpha, epididymis secretory protein Li 17, non-neural enolase, phosphopyruvate hydratase, plasminogen-binding protein, tau-crystallin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06733
  • Protein Name
    Alpha-enolase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A431 whole cell lysate All lanes: ENO1 antibody at 2.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 48, 37 kDa Observed band size: 48 kDa
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Figure 1. Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: monkey kidney tissue lysates, Lane 8: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (Catalog # A01250-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENO1 at approximately 47KD. The expected band size for ENO1 is at 47KD.
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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
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