Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ENO1 antibody [N3C3] (GTX101803) at 1:300 dilution.
![ENO1 antibody [N3C3] detects Eno1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. ENO1 antibody [N3C3] (GTX101803) dilution: 1:100.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101803/GTX101803_41738_20160530_IHC-Wm_Z_22111423_194.webp "ENO1 antibody [N3C3] detects Eno1 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. ENO1 antibody [N3C3] (GTX101803) dilution: 1:100.")
![ENO1 antibody [N3C3] detects ENO1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ENO1 protein stained by ENO1 antibody [N3C3] (GTX101803) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101803/GTX101803_39799_20150410_IFA_w_23060100_918.webp "ENO1 antibody [N3C3] detects ENO1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ENO1 protein stained by ENO1 antibody [N3C3] (GTX101803) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000. Blue: Hoechst 33342 staining.")
![Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101803/GTX101803_39799_20200424_WB_R_lung_w_23060100_281.webp "Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101803/GTX101803_39799_20161103_WB_shRNA_watermark_w_23060100_296.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101803/GTX101803_39799_20200424_WB_R_w_23060100_658.webp "Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [N3C3] (GTX101803) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ENO1 antibody [N3C3] (GTX101803) at 1:300 dilution.
ENO1 antibody [N3C3]
GTX101803
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetENO1
Overview
- SupplierGeneTex
- Product NameENO1 antibody [N3C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2023
- Target nameENO1
- Target descriptionenolase 1
- Target synonymsENO1-IT1, ENO1L1, HEL-S-17, MPB1, NNE, PPH, alpha-enolase, c-myc promoter-binding protein-1, 2-phospho-D-glycerate hydro-lyase, ENO1 intronic transcript 1, ENO1 intronic transcript 1 (non-protein coding), MYC promoter-binding protein 1, alpha enolase like 1, enolase 1, (alpha), enolase-alpha, epididymis secretory protein Li 17, non-neural enolase, phosphopyruvate hydratase, plasminogen-binding protein, tau-crystallin
- HostRabbit
- IsotypeIgG
- Protein IDP06733
- Protein NameAlpha-enolase
- Scientific DescriptionThis gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- De Tomi E, Campagnari R, Orlandi E, et al. Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins. Int J Mol Sci. 2022,23(3). doi: 10.3390/ijms23031647Read this paper
- Chang SJ, Liao EC, Yeo HY, et al. Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma. Arch Biochem Biophys. 2018,647:10-32. doi: 10.1016/j.abb.2018.04.004Read this paper
- Zhou X, Yao K, Zhang L, et al. Identification of Differentiation-Related Proteins in Gastric Adenocarcinoma Tissues by Proteomics. Technol Cancer Res Treat. 2016,15(5):697-706. doi: 10.1177/1533034615595792Read this paper
- Lin LL, Hsia CR, Hsu CL, et al. Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells. BMC Genomics. 2015,16(1):41. doi: 10.1186/s12864-015-1230-0Read this paper
- Wu YH, Hu CW, Chien CW, et al. Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin. PLoS One. 2013,8(8):e70642. doi: 10.1371/journal.pone.0070642Read this paper
- Hsiao KC, Shih NY, Fang HL, et al. Surface α-enolase promotes extracellular matrix degradation and tumor metastasis and represents a new therapeutic target. PLoS One. 2013,8(7):e69354. doi: 10.1371/journal.pone.0069354Read this paper
Datasheet
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