![Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT2217] (GTX630507) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT2217] (GTX630507) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630507/GTX630507_41568_20200424_WB_R_lung_w_23061202_297.webp)
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT2217] (GTX630507) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
ENO1 antibody [GT2217]
GTX630507
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Rat
TargetENO1
Overview
- SupplierGeneTex
- Product NameENO1 antibody [GT2217]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT2217
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2023
- Target nameENO1
- Target descriptionenolase 1
- Target synonymsENO1-IT1, ENO1L1, HEL-S-17, MPB1, NNE, PPH, alpha-enolase, c-myc promoter-binding protein-1, 2-phospho-D-glycerate hydro-lyase, ENO1 intronic transcript 1, ENO1 intronic transcript 1 (non-protein coding), MYC promoter-binding protein 1, alpha enolase like 1, enolase 1, (alpha), enolase-alpha, epididymis secretory protein Li 17, non-neural enolase, phosphopyruvate hydratase, plasminogen-binding protein, tau-crystallin
- HostMouse
- IsotypeIgG2b
- Protein IDP06733
- Protein NameAlpha-enolase
- Scientific DescriptionThis gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]
- ReactivityHuman, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![ENO1 antibody [GT2217] detects ENO1 protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract 10 % SDS-PAGE ENO1 antibody [GT2217] (GTX630507) dilution: 1:10000 ENO1 antibody [GT2217] detects ENO1 protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract 10 % SDS-PAGE ENO1 antibody [GT2217] (GTX630507) dilution: 1:10000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630507/GTX630507_41568_WB_w_23061202_654.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT2217] (GTX630507) diluted at 1:15000. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT2217] (GTX630507) diluted at 1:15000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630507/GTX630507_41568_20161103_WB_shRNA_watermark_w_23061202_564.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT2217] (GTX630507) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT2217] (GTX630507) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630507/GTX630507_41568_20200424_WB_R_w_23061202_618.webp)


![Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ENO1 antibody [N3C3] (GTX101803) at 1:300 dilution.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101803/GTX101803_39799_IHC_Z_22111423_494.webp)

![WB analysis of various samples using GTX57673 ENO1 + ENO2 antibody [AT1G7]. Lane 1 : PC-3 whole cell lysate Lane 2 : MCF-7 whole cell lysate Lane 3 : 293T whole cell lysate Lane 4 : HeLa whole cell lysate Loading : 40 μg Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX57673/GTX57673_20200204_WB2_w_23061123_745.webp)
![Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] (GTX630506) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX630506/GTX630506_41568_20200424_WB_R_lung_w_23061202_309.webp)