More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ENO1 antibody
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:20000.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:20000.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody (GTX113179) diluted at 1:20000.

ENO1 antibody

GTX113179
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetENO1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    ENO1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.26 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2023
  • Target name
    ENO1
  • Target description
    enolase 1
  • Target synonyms
    ENO1-IT1, ENO1L1, HEL-S-17, MPB1, NNE, PPH, alpha-enolase, c-myc promoter-binding protein-1, 2-phospho-D-glycerate hydro-lyase, ENO1 intronic transcript 1, ENO1 intronic transcript 1 (non-protein coding), MYC promoter-binding protein 1, alpha enolase like 1, enolase 1, (alpha), enolase-alpha, epididymis secretory protein Li 17, non-neural enolase, phosphopyruvate hydratase, plasminogen-binding protein, tau-crystallin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06733
  • Protein Name
    Alpha-enolase
  • Scientific Description
    This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Tramutola A, Sharma N, Barone E, et al. Proteomic identification of altered protein O-GlcNAcylation in a triple transgenic mouse model of Alzheimer's disease. Biochim Biophys Acta Mol Basis Dis. 2018,1864(10):3309-3321. doi: 10.1016/j.bbadis.2018.07.017
    Read this paper
  • Kavanagh Williamson M, Coombes N, Juszczak F, et al. Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1. Viruses. 2018,10(3). doi: 10.3390/v10030114
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-Alpha-enolase [EnL5]Ab02940-10.0
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
ReactivityHuman
TargetENO1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ENO1 Antibody144-64672
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetENO1
  • SizePrice
Product group Antibodies
Biosynth
ENO1 antibody70R-12612
  • SizePrice
Product group Antibodies
Abbexa
STRIP1 AntibodyABX126672
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • SizePrice
Figure 1. Western blot analysis of ENO1 using anti-ENO1 antibody (A01250-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: monkey kidney tissue lysates, Lane 8: monkey liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO1 antigen affinity purified polyclonal antibody (Catalog # A01250-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENO1 at approximately 47KD. The expected band size for ENO1 is at 47KD.
Product group Antibodies
Boster Bio
Anti-ENO1 Antibody Picoband(r)A01250-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetENO1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ENO1 antibody [N3C3] (GTX101803) at 1:300 dilution.
Product group Antibodies
References
GeneTex
ENO1 antibody [N3C3]GTX101803
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetENO1
  • SizePrice
WB analysis of various samples using GTX57673 ENO1 + ENO2 antibody [AT1G7]. Lane 1 : PC-3 whole cell lysate Lane 2 : MCF-7 whole cell lysate Lane 3 : 293T whole cell lysate Lane 4 : HeLa whole cell lysate Loading : 40 μg Dilution : 1:1000
Product group Antibodies
GeneTex
ENO1 + ENO2 antibody [AT1G7]GTX57673
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetENO1
  • SizePrice
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT186] (GTX630506) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
ENO1 antibody [GT186]GTX630506
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetENO1
  • SizePrice
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ENO1 antibody [GT2217] (GTX630507) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
ENO1 antibody [GT2217]GTX630507
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetENO1
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-ENO1Y058193
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice