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FACS (Intracellular staining) analysis of RAW264.7 cells using GTX30618 EP300 antibody [RW105]. Blue : Primary antibody Orange : isotype control Dilution : 2 microg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
FACS (Intracellular staining) analysis of RAW264.7 cells using GTX30618 EP300 antibody [RW105]. Blue : Primary antibody Orange : isotype control Dilution : 2 microg/mL Fixation : 4% PFA Permibilization : 0.1% saponin
FACS (Intracellular staining) analysis of RAW264.7 cells using GTX30618 EP300 antibody [RW105]. Blue : Primary antibody Orange : isotype control Dilution : 2 microg/mL Fixation : 4% PFA Permibilization : 0.1% saponin

EP300 antibody [RW105]

GTX30618
GeneTex
ApplicationsElectron Microscopy, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, Other Application
Product group Antibodies
TargetEP300
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Overview

  • Supplier
    GeneTex
  • Product Name
    EP300 antibody [RW105]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:250 - 1:500. ICC/IF: 1:50 - 1:200. IP: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Electron Microscopy, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RW105
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2033
  • Target name
    EP300
  • Target description
    E1A binding protein p300
  • Target synonyms
    E1A-associated protein p300; E1A-binding protein, 300kD; histone acetyltransferase p300; histone butyryltransferase p300; histone crotonyltransferase p300; KAT3B; MKHK2; p300; p300 HAT; protein 2-hydroxyisobutyryltransferase p300; protein lactyltransferas p300; protein propionyltransferase p300; RSTS2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ09472
  • Protein Name
    Histone acetyltransferase p300
  • Scientific Description
    This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Thyroid Hormone Induces Oral Cancer Growth via the PD-L1-Dependent Signaling Pathway.
    Read more

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Figure 1. Western blot analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/P300/EP300 antigen affinity purified polyclonal antibody (Catalog # A00117) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT3B/P300/EP300 at approximately 264-300 kDa. The expected band size for KAT3B/P300/EP300 is at 264 kDa.
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