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ProductsBack/Eph receptor A3 (EPHA3) (N-term) Rabbit Polyclonal Antibody
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Eph receptor A3 (EPHA3) (N-term) Rabbit Polyclonal Antibody

AP14276PU-N
OriGene
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEPHA3
Price on request
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Eph receptor A3 (EPHA3) (N-term) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2042
  • Target name
    EPHA3
  • Target description
    EPH receptor A3
  • Target synonyms
    EK4, ETK, ETK1, HEK, HEK4, TYRO4, ephrin type-A receptor 3, EPH-like kinase 4, TYRO4 protein tyrosine kinase, eph-like tyrosine kinase 1, human embryo kinase 1, testicular tissue protein Li 64, tyrosine-protein kinase receptor ETK1
  • Host
    Rabbit
  • Protein IDP29320
  • Protein Name
    Ephrin type-A receptor 3
  • Scientific Description
    Eph receptor A3 (EPHA3) (N-term) rabbit polyclonal antibody, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eph Receptor A3/EPHA3 using anti-Eph Receptor A3/EPHA3 antibody (A02872-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph Receptor A3/EPHA3 antigen affinity purified polyclonal antibody (Catalog # A02872-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph Receptor A3/EPHA3 at approximately 135 kDa. The expected band size for Eph Receptor A3/EPHA3 is at 110 kDa.
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Lane 1: H9C2 cell lysates probed with Eph receptor A3 Polyclonal Antibody, Unconjugated (bs-7032R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with EphA3 antibody [N1N3] (GTX114067) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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